This document is a new user guide for Leica Microsystems' SP5 laser scanning microscope, which explains the steps for system startup, laser activation, and image acquisition settings.
This guide walks new users through the basic steps to start the Leica TCS SP5 system, activate lasers, and configure image acquisition settings. It includes instructions for laser activation, beam‑path setup, and gain adjustment to obtain clear fluorescence images.
Additional sections cover advanced functions such as Z‑stack acquisition, 3‑D projection creation, and movie generation, helping users to capture and visualize volumetric data from their samples.
| Image format | 512 x 512 pixels |
|---|---|
| Scan speed | 400 Hz |
| Initial laser intensity | AOTF % 20‑30 % |