Industrial

Agilent Poroshell 300SB-C3 is a unique microparticulate superficially-porous C3 column packing from Agilent. Columns of this material are especially useful for carrying out ultra-fast gradient separations of macromolecules because of the favorable kinetic properties of the special silica support. Very rapid gradient separation of proteins, poly-peptides and related bio-macromolecules is attained by operating these columns at high flow rates (mobile phase velocities); rapid mass transfer maintains good column efficiency for excellent separations. This StableBond (SB) packing is made by covalently bonding a patented sterically-protected C3 stationary phase to the specially prepared, high-purity Poroshell 300 microparticles. The special silica support is designed to reduce or eliminate strong adsorption of basic compounds, and is especially friendly towards biomolecules such as proteins. The densely covered, sterically-protected triisopropylsilane stationary phase allows these particles to be routinely used at low pH (for example, pH 1) and temperatures to at least 70 °C with excellent stability. Therefore, a Poroshell 300SB-C3 is particularly well suited for use with aggressive mobile phases that are widely used for the reversed-phase separation of proteins and other such biomacromolecules (for example, pH < 2, high ionic strength, ion-pair additives, and so forth). This packing also is useful for separating compounds such as proteins.

VariTide RPC Media is chemically and physically stable across the complete pH range. This guide is intended to help with column packing, performance and lifetime.

Agilent 7000B Triple Quadrupole GC/MS System Data Sheet is a high-performance gas chromatography mass spectrometry (GC/MS) instrument with outstanding sensitivity and selectivity. The system consists of a 7000B GC/MS triple quadrupole mass spectrometer and MassHunter software. The GC and MS components have been designed to deliver consistent sensitivity and accurate quantification of trace-level target compounds in very complex matrices.

The Agilent 4500 Series FTIR spectrometers are mid-IR spectrometers specifically designed for analysis outside of laboratory environments. The system is compact and packaged in a weather-resistant housing making it ideal for outdoor use.

The Agilent 2200 TapeStation system accelerates electrophoretic analysis of RNA, DNA and proteins, and it offers scalable throughput and rapid results. The system is an ideal solution for quality control of biological samples in next-generation sequencing (NGS), microrarray and qPCR workfl ows, as well as in protein purifi cation and antibody production. The 2200 TapeStation system uses credit card-sized, disposable ScreenTape that is available for DNA, RNA and protein applications. Gel analysis has never been so easy – simply load the 2200 TapeStation instrument with ScreenTape, loading tips, and your samples in 16-tube strips or 96-well plates – and you will be reviewing results in about 1 minute per sample!

The Agilent D1K ScreenTape and High Sensitivity D1K ScreenTape accelerate DNA analysis with the Agilent 2200 TapeStation system. The system provides automated, fast and reliable RNA, DNA and protein electrophoresis. The D1K ScreenTape has been developed for the separation and analysis of DNA fragments from 35 bp to 1000 bp. Depending on the sensitivity requirements, it is possible to choose between the D1K ScreenTape and the High Sensitivity D1K ScreenTape. DNA sample analysis has never been so easy - simply load the 2200 TapeStation with the appropriate D1K ScreenTape, loading tips, and samples in tube strips or 96-well plates, and you will be reviewing results in 1 minute per sample.

The Helijet by Agilent Technologies is specifically designed for cryo-crystallography experiments requiring temperatures in the range of 75-15 Kelvin. It is developed for use with Agilent Technologies' Xcalibur, Gemini, and SuperNova diffractometers, and its compact construction is ideal for CCD diffractometers. The unique design of the Helijet allows for complete freedom of movement of a Kappa goniometer and unobscured optical access to the biological macromolecular or small molecule crystal without the need for crystal shielding. The Helijet provides an efficient cold stream up to 5 mm from the nozzle tip with a very uniform temperature distribution within the cold stream. To prevent ice formation, the Helijet utilizes shield gas technology. It has a patented design that minimizes helium consumption to less than 2 liters per hour and achieves a base temperature of less than 10 K.

The Agilent 7890A is a state-of-the-art gas chromatograph that provides superior performance for all applications. Key to its performance is the use of advanced electronic pneumatic control (EPC) modules and high performance GC oven temperature control. The combination of precise pneumatic and temperature control leads to extremely precise retention time reproducibility, which is the basis for all chromatographic measurement. Agilent’s proprietary Capillary Flow Technology provides a new dimension in chromatography with reliable, leak-free, in-oven capillary connections that stand up to repeated GC oven cycling over time. The 7890A GC has enhanced firmware to extend Capillary Flow capabilities and enhanced datasystem software to simplify set-up and operation of backflush. These new tools make it easier to analyze complex matrices and unknowns, and provide gains in productivity and data integrity for routine analyses via 2-dimensional heart cutting, detector splitting, and backflush.

Agilent 800 MHz PremiumCOMPACT NMR Magnet is a new type of superconducting NMR magnet. It uses the latest superconducting wire technology to operate at 4.2 Kelvin, which saves more laboratory space and is more convenient to use than traditional 800 MHz magnets. It also has lower operating costs.

AffinityScript One-Step RT-PCR Kit is a high-performance single-step RT-PCR kit that can achieve full-length, high-fidelity RT-PCR for targets up to 9.5 kb in length and obtain high yields. This kit is suitable for gene profiling and other high-throughput end-point RT-PCR applications due to its high throughput and rapid running.

Agilent 1260 Infinity Bio-inert Quaternary LC is a dedicated solution for large bio-molecule analysis. The design of new metal-free components in the sample flow-path and the absence of iron and steel in solvent delivery ensures the integrity bio-molecule, minimizes unwanted surface interactions and increases column life-time. This is ideal when working under harsh solvent or pH conditions. The power ranges from lowest pressure for traditional bio-purification columns up to high pressure STM analytical bio-columns. Together with the new Bio-HPLC column portfolio for SEC and IEX and 10x higher sensitivity, highest resolution per time is achieved for protein and NBE characterization.

This data sheet introduces the Agilent Protein Fractionation System and Proteomics Reagents for LC/MS analysis of biological samples, including antibody depletion, sample desalting, concentration, and fractionation.