Agilent Manuals (Industrial)

This article introduces a flexible analytical tool for directly analyzing the trace element content of solid samples. Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) enables identification and comparison of physical evidence, discriminating elemental and isotopic differences at the part per billion (ppb) level.

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Application of Collision/Reaction Cell ICP-MS to the Analysis of Variable and Unknown Samples Without Requiring Matrix-Specific or Element-Specific Tuning and Cell Conditions Cell Operation with an Inert Cell Gas Using an inert cell gas and efficient energy discrimination (ED) removes all of these reported problems, since the cell is operating as a non-reactive environment. The use of an inert gas allows ED to be used as the primary interference removal process, which eliminates the method development inherent in the use of reactive cell gases. This means that the analysis conditions are independent of the analyte being measured (and of the interferences being removed), multi- element analysis is possible and the operating conditions do not have to be optimised for each matrix type. A further benefit of collision mode is that no interference correction equations are necessary. Use of ED depends on the use of a high purity inert cell gas, which is free from organic contaminants and water vapour, so plumbing of the gas supply requires care. A light collision gas (He) is preferred, since less analyte ion scattering occurs. Introduction. ICP-MS is a multi-element analytical technique, which uses a high temperature plasma source to decompose, dissociate, atomise and ionise the elements of interest in a sample, providing high sensitivity, rapid analysis, low detection limits, simple spectra and routine operation. In simple solutions, few interferences obscure the measurement of the

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This application brief describes a method for the semiquantitative analysis of glass fragments using laser ablation ICP-MS. The method can rapidly and accurately identify glass fragments, and requires no other chemical reagents.

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This application note describes the determination of vanadium and nickel in heavy oil by ICP-MS using the Agilent 4500 ICP-MS

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Semiquantitative Analysis of Glass by Laser Ablation ICP-MS

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Using ICP-MS as a Rapid Survey Tool for Semiquantitative Analysis

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This paper introduces a method for implementing precise time-scaled versions of a method on multiple instrument types using method translation combined with retention time locking (RTL). This method allows the original method to be reused with minimal effort, while optimizing the method for a given sample type or instrument setup.

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This application note describes how to use method translation combined with RTL to implement precise time-scaled versions of a method on multiple instru- ment types.

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This document introduces CyberPrinter XP, which is a print management system that manages the transfer of human readable electronic documents to Cerity ECM. The system works with third-party print drivers from any operating system and allows users to upload electronic records generated during a print operation from any application. CyberPrinter XP can manage files generated by one or more printers, providing the user with a full suite of printing and file upload options.

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This Application Note describes different flow splitter designs and configurations used in high-purity separations.

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Simultaneous assessment of drug metabolic stability and identification of metabolites using HPLC-Chip/ion trap mass spectrometry can effectively identify metabolites, save time and improve work efficiency

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In this study, an established HPLC method for USP Pheno-barbital was sequentially and quickly improved to a high throughput method using Rapid Resolution (RR) and Rapid Resolution High Throughput (RRHT) techniques. New RRHT column technology is an especially powerful tool for improving lab productivity by reducing HPLC analysis time dramatically. Starting with a proven rugged method, conversion to a high throughput method can be achieved simply by replacing the original analytical-sized column with either a RR or RRHT column. The reasons for this direct conversion include the similar selectivity of smaller 1.8- and 3.5-µm particles compared to larger 5-µm particles, and an engineered particle size distribution that reduces unacceptably high system pressures that may be experienced with other sub-two micron packings.

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This is an application note on a drug called pindolol. It is used to lower blood pressure and alleviate depression. The note describes a method for conducting HPLC separation using pindolol and USP method. It also describes a method for conducting HPLC separation using sub-2-micron support particles (ZORBAX SB-CN (L10) columns).

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Automated software-guided identification of new buspirone metabolites using capillary LC coupled to ion trap and TOF mass spectrometry

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The present application note describes a method for the analysis of small RNAs using the Agilent 2100 bioanalyzer. The method involves the use of the Total RNA 6000 Nano Kit to extract small RNAs, and then the use of the bioanalyzer to separate, detect, and quantify the RNAs. The results showed that the method is highly sensitive and accurate, and is suitable for the rapid and accurate analysis of small RNAs.

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This article describes the analysis of a complex natural product extract from ginseng. By using LC/MS technology, it is possible to differentiate between different species of ginseng plants and authenticate ginseng products.

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Determination of Toxic Elements in Traditional Chinese Medicine Using Inductively Coupled Plasma Mass Spectrometry

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This Application Note describes a method for studying noncovalent protein interactions using the HPLC-Chip/MS system coupled with an ESI-TOF mass spectrometer. The application note studies apomyoglobin and myoglobin protein complexes. The results show that ESI/MS is an efficient and accurate method for studying noncovalent protein interactions.

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Fast and Ultra-fast Analysis with the Agilent 1200 Series Rapid Resolution LC System Compared to a Conventional Agilent 1100 Series LC System Using Sub 2-µm Particle Columns

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This application note describes the application of the Low RNA Input Fluorescent Linear Amplification kit for the use of poly (A)+ RNA or total RNA samples. It shows that micrograms of cRNA targets can be generated within 6 hours, using as little as 10 ng of poly (A)+ RNA, or 50 ng of total RNA sample input. The resulting cRNA targets were of high quality and per- formed reproducibly and robustly on Agilent's oligonu- cleotide microarrays. Furthermore, the log ratios of array results from poly (A)+ RNA sample input correlated with that from total RNA input. The easy-to-use Low RNA Input Fluorescent Linear Amplification kit can be applied to a wide range of samples for gene expression analysis.

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