Update: 30 September, 2023
This application note describes the application of the Low RNA Input Fluorescent Linear Amplification kit for the use of poly (A)+ RNA or total RNA samples. It shows that micrograms of cRNA targets can be generated within 6 hours, using as little as 10 ng of poly (A)+ RNA, or 50 ng of total RNA sample input. The resulting cRNA targets were of high quality and per- formed reproducibly and robustly on Agilent's oligonu- cleotide microarrays. Furthermore, the log ratios of array results from poly (A)+ RNA sample input correlated with that from total RNA input. The easy-to-use Low RNA Input Fluorescent Linear Amplification kit can be applied to a wide range of samples for gene expression analysis.
File format: PDF
Size: -
MD5 Checksum: 2280CB22DC6653E48425EAC6F21A7309
Publication date: 02 July, 2012
Downloads: -
PDF Link: Agilent Using Low Input of Poly (A) RNA Total RNA for Oligonucleotide Microarrays PDF