Update: 28 September, 2023
In this study, an established HPLC method for USP Pheno-barbital was sequentially and quickly improved to a high throughput method using Rapid Resolution (RR) and Rapid Resolution High Throughput (RRHT) techniques. New RRHT column technology is an especially powerful tool for improving lab productivity by reducing HPLC analysis time dramatically. Starting with a proven rugged method, conversion to a high throughput method can be achieved simply by replacing the original analytical-sized column with either a RR or RRHT column. The reasons for this direct conversion include the similar selectivity of smaller 1.8- and 3.5-µm particles compared to larger 5-µm particles, and an engineered particle size distribution that reduces unacceptably high system pressures that may be experienced with other sub-two micron packings.
File format: PDF
Size: -
MD5 Checksum: 0619E568DF8C3AF73602A5E4354C3BE4
Publication date: 02 July, 2012
Downloads: -
PDF Link: Agilent High Throughput HPLC Analysis of Barbiturates Application Note PDF