This document details the composition, customer-prepared reagents, culture conditions, culture methods, and common problems and solutions for the SGC7901/DDP human gastric cancer cisplatin-resistant strain.
The SGC7901/DDP cell line is a cisplatin‑resistant gastric cancer strain supplied by Bogu Biotechnology for research use only. The manual outlines the cell composition, necessary reagents, and detailed culture conditions.
It describes the step‑by‑step culture method, including drug‑containing medium preparation, passage procedures, and common problems with solutions such as handling bottle leaks or floating cells. This manual is written in Chinese.
| Product number | HDRC012 |
|---|---|
| Culture temperature | 37.0°C |
| CO₂ concentration | 5% |
| Culture medium | RPMI1640+10%CS+P/S+DDP 1000 ng/ml |
The required reagents are PBS, a suitable culture medium, and 0.25 % (W/V) trypsin‑EDTA (containing 0.53 mM EDTA).
Wipe the bottle mouth with alcohol, lay the bottle flat in the incubator, and observe. If cells remain floating, centrifuge them and re‑seed the pellet into fresh medium.