MAP-2 ELISA kit instruction manual, which can be used to quantitatively measure the content of MAP-2 in rat serum, plasma, tissue homogenate, cell culture supernatant or other related liquids.
This manual describes the ELISA method for quantifying MAP‑2 protein in samples such as serum, plasma, and cell‑culture supernatant. The assay uses a sandwich format with a solid‑phase antibody, biotin‑labeled detection antibody, and HRP‑TMB color development, measured at 450 nm.
Detailed procedures cover reagent reconstitution, plate loading, incubation times, washing steps, and data analysis. Storage conditions for reagents and samples are also provided, along with precautions to ensure reliable results. This manual is written in Chinese.
| Detection range | 0.156 ng/mL – 10 ng/mL |
|---|---|
| Lower limit of detection | 0.078 ng/mL |
| Reagent storage temperature | -20 °C |
The kit can detect MAP‑2 concentrations from 0.156 ng/mL up to 10 ng/mL.
All reagents should be stored at –20 °C when not in use.