The rat α1 microglobulin bikunin precursor (AMBP) ELISA kit is used for in vitro quantitative measurement of AMBP content in rat serum, plasma, tissue homogenate, cell culture supernatant or other related liquids.
The manual describes the ELISA principle based on a sandwich immunoassay using a capture antibody coated micro‑plate, biotin‑labeled detection antibody, and HRP‑TMB color development. It explains how to prepare standards, samples (serum, plasma, cell culture supernatant) and the required reagents.
Detailed procedures include adding standards and samples to wells, incubation times, washing steps, addition of substrate solution, and measurement of absorbance at 450 nm. The document also provides guidance on reagent storage, plate handling, and troubleshooting tips to ensure reliable results. This manual is written in Chinese.
| Detection range | 0.156 ng/mL – 10 ng/mL |
|---|---|
| Measurement wavelength | 450 nm |
| Reagent storage temperature | -20 °C (2–8 °C for frequent use) |
The assay can quantify AMBP concentrations from 0.156 ng/mL up to 10 ng/mL.
Read the absorbance at 450 nm after the color development step.
Store the reagents at –20 °C; for frequent use keep them at 2–8 °C.