GENMED QuikFusin Non-Lipid Cell Transfection Reagent is a high-efficiency, low-toxic non-lipid lipid-mediated carrier that does not require serum removal, can form a complex with the target DNA, and is transferred into cells. A classic technology with authoritative and proven technology. It is suitable for various fragments including large fragments of DNA (>12 kb), oligonucleotides, etc. and can be transferred into more than 700 types of adherent and suspended cell lines (strains). It can be used for stable transfection or transient transfection. The product is strictly sterile, ready-to-use, free of nuclease contamination, optimized proportion, simple operation, stable performance, and significant transfection efficiency.
The QuikFusin kit is a non‑liposomal transfection reagent designed for high‑efficiency, low‑toxicity delivery of DNA fragments (including >12 kb) and oligonucleotides into over 700 adherent and suspension cell lines. The kit includes Reagent A (transfection solution) and Reagent B (mediating solution) that are mixed immediately before use.
The manual outlines the preparation of the transfection mixture, the recommended cell density (≈1 × 10⁶ cells per 25 cm² flask), DNA amount (7–9 µg), incubation times (15 min at room temperature, then 3 h at 37 °C), and post‑transfection handling, including optional selection steps for stable expression. Storage, quality standards, and ordering information are also provided. This manual is written in Chinese.
| Number of transfections per kit | 10 |
|---|---|
| DNA amount per transfection | 7–9 µg |
| Cell number per transfection | 1 × 10⁶ cells |
One kit provides enough reagents for 10 transfections.
Use 7 to 9 µg of DNA for each transfection.
Incubate the mixture at room temperature for 15 minutes.
Place the cells in a 37 °C incubator after adding the mixture.