This document is the instruction manual for the Rat Hepcidin Enzyme-Linked Immunosorbent Assay (ELISA) Kit, which is used to measure the level of Hepcidin in rat serum, plasma, and other related liquid samples. The kit utilizes a sandwich immunoassay method with purified rat Hepcidin antibody coated on the microplate. The resulting antibody-antigen-enzyme conjugate complex is detected using a colorimetric substrate and measured at 450nm wavelength with a microplate reader to determine the concentration of rat Hepcidin in the samples.
This manual describes the principle, reagents, and step‑by‑step procedure for the rat Hepcidin ELISA kit. The assay uses a double‑antibody sandwich format with HRP‑labeled detection antibody and TMB substrate, and results are read at 450 nm.
The document includes reagent composition, sample requirements, incubation conditions, washing steps, color development, termination, calculation of concentrations, and safety and storage guidelines. This manual is written in Chinese.
| Sample Types | Rat serum, plasma, and related liquid samples |
|---|---|
| Assay Principle | Double‑antibody sandwich ELISA |
| Detection Range | 0‑100 µg/L |
| Incubation Temperature | 37 °C |
| Shelf Life | 6 months |
| Storage Conditions | Refrigerated; equilibrate to room temperature 15‑30 min before use |
The kit can measure Hepcidin in rat serum, plasma, and related liquid samples.
It uses a double‑antibody sandwich ELISA format with HRP‑labeled detection antibody.
Incubate the sealed plate at 37 °C for 30 minutes.
Keep the kit refrigerated and allow it to reach room temperature for 15‑30 minutes before use.