This is a product description document that introduces RayBio® Mouse IL-33 IQELISA Kit. This kit is an innovative immuno-quantitative enzyme-linked immunosorbent assay (IQELISA) that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. The kit can be used to quantify mouse IL-33 in serum, plasma and cell culture supernatants.
This document provides step-by-step instructions for using the RayBio® Mouse IL-33 IQELISA Kit, a modified ELISA assay combining PCR sensitivity with ELISA specificity. It includes sections on reagent preparation, assay procedure, data calculation, and troubleshooting. The kit requires a real-time PCR instrument and specific reagents for accurate quantification of Mouse IL-33.
The assay involves coating a PCR plate with anti-Mouse IL-33 antibody, adding standards and samples, incubating, washing, adding detection reagents, and running qPCR to obtain Cq values for concentration calculation.
| Catalog Number | IQM-IL33 |
|---|---|
| PCR Instrument Requirement | Real-time PCR instrument (Bio-Rad recommended) |
| Detection Wavelength | FITC compatible wavelength |
Serum, plasma, and cell culture supernatants.
The catalog number is IQM-IL33.
The kit can be stored at -20°C for up to one year.
The suggested dilution range is 2-20 fold.