RayBio® Mouse IL-1 beta IQELISA Kit is a modified ELISA assay with high sensitivity qPCR readout for the quantitative measurement of Mouse IL-1b in serum, plasma, and cell culture supernatants.
The RayBiotech Mouse IL-1 beta IQELISA Kit is a modified ELISA assay with a high-sensitivity qPCR readout designed for the quantitative measurement of Mouse IL-1b. The kit includes a 96-well PCR plate coated with anti-Mouse IL-1b, wash buffers, recombinant standards, and reagents for detection and PCR amplification. It is intended for use in serum, plasma, and cell culture supernatants.
| Minimum quantifiable dose | 1.95 pg/ml |
|---|---|
| Serum spike recovery | 80% (range 72% to 86%) |
| Intraplate CV | below 10% |
| Interplate CV | below 15% |
| Storage temperature (kit) | 2° to 8°C |
| Storage temperature (Standard) | -20°C or -80°C |
The minimum quantifiable dose is typically 1.95 pg/ml.
The Standard should be stored at -20°C or -80°C after reconstitution.
Standards and samples should be incubated for 1.5 - 2.5 hours at room temperature.