The Thermo Fisher Scientific Sf-900™ II SFM User Manual provides instructions for preparation, subculture, adaptation, and cryopreservation of insect cell…
This manual describes the complete, serum‑free, protein‑free Sf-900™ II SFM medium and its optimized components for high‑density insect cell growth using the Baculovirus Expression Vector System. It lists product catalog numbers, storage conditions, and safety information.
Detailed protocols cover recovery of frozen cells, subculture of suspension and monolayer cultures, direct and sequential adaptation to the medium, and cryopreservation procedures. Additional information includes related products, symbols, and licensing details.
| Storage temperature | 2°C to 8°C |
|---|---|
| Shelf life | 12 months |
| Culture temperature range | 27°C to 28°C |
| Incubator atmosphere | Non‑humidified, air‑regulated non‑CO₂ |
Store the medium at 2 °C to 8 °C and protect it from light.
Incubate cultures at 27 °C to 28 °C.
Subculture cells a minimum of three passages before downstream use.
The medium remains stable for 12 months when stored properly.