User guide for Thermo Fisher's Qubit 1X dsDNA High Sensitivity assay kit, covering preparation, measurement and handling.
The Qubit 1X dsDNA HS Assay Kit provides a high‑sensitivity method for quantifying double‑stranded DNA. The kit includes a ready‑to‑use working solution, two DNA standards and required consumables. Samples (1–20 µL) are mixed with the working solution and read on a Qubit Fluorometer, delivering a detection range of 0.2 ng to 100 ng of DNA.
The guide details critical assay parameters such as temperature (18–28 °C), incubation time (2 min), photostability, and calibration procedures for both the Qubit Flex and Qubit 3/4 Fluorometers. It also lists compatible assay kits, contaminant tolerances, storage conditions, and ordering information.
| Detection range | 0.2 ng to 100 ng of DNA |
|---|---|
| Sample volume | 1–20 µL |
| Storage temperature | 2–8 °C, protected from light |
| Shelf life (when stored as directed) | at least 6 months |
| Signal stability after reading | up to 3 hours when protected from light |
The assay accepts sample volumes from 1 µL up to 20 µL.
The fluorescence signal remains stable for up to three hours when the tubes are protected from light.
The assay tolerates salts, free nucleotides, solvents, detergents, proteins and several other contaminants at the concentrations listed in Table 2.