The guide provides protocols, guidelines, and troubleshooting for using the ProtoArray® Human Protein Microarray v5.1 in various research applications.
The ProtoArray® Human Protein Microarray v5.1 contains over 9,000 purified human proteins printed on a nitrocellulose‑coated glass slide. It supports multiple assay types, including protein‑protein interaction, kinase substrate identification, small‑molecule profiling, ubiquitin‑ligase profiling, immune‑response biomarker profiling, and antibody specificity profiling.
The guide details experimental overviews, step‑by‑step procedures, scanning and data‑analysis methods, expected results, and troubleshooting tips for each application. It also describes array specifications, storage conditions, and available software tools such as the ProtoArray® Prospector.
| Array dimensions | 1 inch × 3 inch (25 mm × 75 mm) |
|---|---|
| Slide material | Glass slide coated with thin‑film nitrocellulose |
| Total subarrays | 48 (4 columns × 12 rows) |
| Subarray size | 4,400 µm × 4,400 µm |
| Spot diameter | ~110 µm |
| Spot spacing | 200 µm center‑to‑center |
| Replicates per sample | 2 |
| Number of human proteins | >9,000 |
| Protein tags | Primarily GST‑tagged; some polyhistidine‑ or IgG‑Fc‑tagged |
Store the microarray at –20 °C after receipt.
Each array contains more than 9,000 human proteins.
Most proteins are GST‑tagged; some are polyhistidine‑ or IgG‑Fc‑tagged.
Each subarray measures 4,400 µm by 4,400 µm.