Thermo Fisher Scientific QuantStudio Design and Analysis Software User Manual

About this manual

This manual provides step‑by‑step instructions for using the QuantStudio™ Design and Analysis desktop software (v1.2.x). It explains how to create, configure, and run experiments, import sample definitions, and manage reagents for the QuantStudio 3 and 5 Real‑Time PCR instruments.

The guide also details result review, plotting options, quality‑control checks, and exporting data. Appendices give shortcuts, supplemental information on instrument power‑on and file transfer, and a list of compatible parts and consumables.

What's inside

• General procedures to set up and run experiments
• General procedures to review results
• Getting started with Standard Curve experiments
• Getting started with Relative Standard Curve and Comparative CT experiments
• Getting started with Genotyping experiments
• Getting started with Presence/Absence experiments
• Getting started with Melt Curve experiments
• Appendix A – Alternative procedures and shortcuts
• Appendix B – Supplemental information
• Appendix C – Parts and materials

Specifications

Frequently asked questions

How do I create a new experiment in the software?

On the Home screen click **Create New Experiment** to start a fresh experiment without pre‑existing settings.

Where are the software templates stored?

Templates are installed in the folder **Program Files (x86)\Applied BioSystems\QuantStudio Design & Analysis Software\templates**.

How can I export experiment results?

Use the **Export experiments or results** option in the Export menu to save data and analysis outputs.