This user guide provides instructions for using the Qubit dsDNA HS Assay Kits (Cat. No. Q32851, Q32854) to accurately quantify double-stranded DNA…
The document describes the Thermo Fisher Scientific Qubit™ dsDNA HS Assay Kits, which are designed for accurate and easy quantitation of double-stranded DNA (dsDNA). The kits include reagents, buffers, and standards compatible with Qubit™ Fluorometers. They offer a high-sensitivity assay range from 5 pg/µL to 120 ng/µL and are tolerant to common contaminants such as salts, free nucleotides, solvents, detergents, or protein.
The guide details the required materials, critical assay parameters like temperature control, and step-by-step protocols for preparing samples and standards. It also covers calibration procedures using Standard #1 (0 ng/µL) and Standard #2 (10 ng/µL), as well as instructions for reading results on Qubit™ Fluorometers.
| Assay Range | 5 pg/µL to 120 ng/µL |
|---|---|
| Assay Range (Total) | 0.1–120 ng |
| Incubation Time | 2 minutes |
The assay is accurate for initial sample concentrations from 5 pg/µL to 120 ng/µL, providing a total range of 0.1–120 ng.
You must incubate the tubes for 2 minutes after mixing the sample or standard with the working solution to allow optimal fluorescence.
Yes, this assay kit can be used with any Qubit™ Fluorometer.
Yes, common contaminants such as salts, free nucleotides, solvents, detergents, or protein are well tolerated in the assay.