This user guide provides procedural guidelines for using irradiated CF1 Mouse Embryonic Fibroblasts (MEFs) as feeder cells to support the culture of human…
This document serves as a user guide for Irradiated CF1 Mouse Embryonic Fibroblasts (MEFs), catalog numbers A34180 and A34181, manufactured by Thermo Fisher Scientific. These feeder cells are isolated from outbred CF1 mice, expanded for up to three passages, and mitotically inactivated by gamma irradiation to support the culture of healthy undifferentiated human and mouse embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs). The guide details storage requirements, necessary materials, and specific culture conditions including media composition, temperature, and atmosphere.
| Cell Source | outbred CF1 mice |
|---|---|
| Passage Limit | up to three passages |
| Inactivation Method | gamma irradiation |
| Storage Temperature | Liquid nitrogen (vapor phase) |
| Culture Temperature | 36°C to 38°C |
| Incubator Atmosphere | 5% CO2 in air |
| Cell Density Recommendation | 3.0 × 104 MEFs/cm2 |
The catalog numbers are A34180 and A34181.
The guide recommends 3.0 × 104 MEFs/cm2 as a good starting point.
Coated vessels can be stored at room temperature for up to 24 hours.