User manual for Takara Bio's SMART-Seq HT PLUS Kit, detailing protocols for cDNA synthesis and Illumina library preparation.
The SMART-Seq HT PLUS Kit (Cat. Nos. R400748 & R400749) enables generation of high‑quality Illumina‑compatible libraries from 1–100 intact cells or ultra‑low amounts of total RNA (10 pg–1 ng). The kit combines reverse transcription and PCR in a single step, followed by a streamlined two‑step library preparation workflow.
The manual provides detailed instructions on required equipment, component handling, contamination prevention, cDNA synthesis, library preparation, quality control, and troubleshooting. It also lists all kit components, additional materials, and recommended storage conditions.
| Reactions per kit | 48 (R400748) or 96 (R400749) |
|---|---|
| Freeze‑thaw limit | ≤6 times |
| Control Total RNA storage | –70 °C |
| Other components storage | –20 °C |
The kit can prepare up to 48 reactions (catalog R400748) or 96 reactions (catalog R400749).
Control Total RNA should be stored at –70 °C, while all other kit components should be stored at –20 °C.
Set the lid temperature to 105 °C if the cycler does not adjust automatically.
The kit works with 10 pg to 1 ng of total RNA; 10 pg is recommended for single‑cell equivalents.