The manual provides description, components, storage, protocol and troubleshooting for the Takara Bio RR096A One Step TB Green PrimeScript PLUS RT‑PCR Kit.
This product manual describes the One Step TB Green® PrimeScript™ PLUS RT‑PCR Kit (catalog number RR096A). It includes a brief description of the kit, the principle of one‑step real‑time RT‑PCR, a list of components, storage conditions, key features, safety precautions, detailed protocols for several real‑time PCR instruments, an experimental example, and related products.
The kit is intended for research use only and is compatible with a range of real‑time PCR platforms such as Applied Biosystems 7300/7500, LightCycler, Smart Cycler, and Thermal Cycler Dice systems. Proper handling, storage at –20 °C, and careful preparation of master mixes are emphasized to ensure reliable results.