The Takara Bio PrimeSTAR* Max DNA Polymerase manual provides usage instructions, optimal PCR parameters, and storage guidelines.
This manual describes the PrimeSTAR* Max DNA Polymerase, a high‑speed, high‑accuracy enzyme for PCR applications. It explains the enzyme’s hot‑start formulation, recommended reaction components, and handling precautions.
Detailed sections cover recommended PCR cycling conditions, optimal template amounts, annealing temperatures, extension times, and troubleshooting tips, along with safety and licensing information for research‑use only. This manual is written in Japanese.
| Mg2+ concentration | 2 mM (2 X) |
|---|---|
| Denaturation step | 98 °C for 10 sec |
| Annealing temperature | 55 °C |
| Extension rate | 5 sec per kb |
Use 2 mM Mg2+ (2 X) as specified in the manual.
The recommended annealing temperature is 55 °C.
Set the extension time to 5 seconds per kilobase.