Key facts
- Manuals
- 5
- Languages
- EN · JA · ZH
- Last added
- 2026-10-01
Featured Takara Bio manuals
Takara Bio RR096A One Step TB Green PrimeScript PLUS RT-PCR Kit User Manual
This product manual describes the One Step TB Green® PrimeScript™ PLUS RT‑PCR Kit (catalog number RR096A). It includes a brief description of the kit, the principle of one‑step real‑time RT‑PCR, a list of components, storage conditions, key features, safety precautions, detailed protocols for several real‑time PCR instruments, an experimental example, and related products.
The kit is intended for research use only and is compatible with a range of real‑time PCR platforms such as Applied Biosystems 7300/7500, LightCycler, Smart Cycler, and Thermal Cycler Dice systems. Proper handling, storage at –20 °C, and careful preparation of master mixes are emphasized to ensure reliable results.
Covers: Description · Principle · Components · Storage · Features · Precautions
Takara Bio SMART-Seq HT PLUS Kit User Manual
The SMART-Seq HT PLUS Kit (Cat. Nos. R400748 & R400749) enables generation of high‑quality Illumina‑compatible libraries from 1–100 intact cells or ultra‑low amounts of total RNA (10 pg–1 ng). The kit combines reverse transcription and PCR in a single step, followed by a streamlined two‑step library preparation workflow.
The manual provides detailed instructions on required equipment, component handling, contamination prevention, cDNA synthesis, library preparation, quality control, and troubleshooting. It also lists all kit components, additional materials, and recommended storage conditions.
Covers: Introduction · List of Components · Additional Materials Required · General Considerations · Sample Recommendations and Requirements · Diluting the Control RNA
Takara Bio SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing User Manual
This manual describes the SMART‑Seq® v4 Ultra Low Input RNA Kit for Sequencing, a kit that generates high‑quality, full‑length cDNA from very small numbers of cells or low amounts of total RNA. It outlines the required reagents, recommended equipment, and clean‑room practices needed to obtain reliable results.
The document is organized into sections covering the list of components, additional materials, general considerations, detailed protocols for first‑strand cDNA synthesis, LD‑PCR amplification, bead purification, validation, and library preparation for Illumina and Ion Torrent platforms, followed by references and appendices for magnetic bead handling and PCR optimization.
Covers: Introduction · List of Components · Additional Materials Required · General Considerations · Protocols · Library Preparation for Sequencing
Takara Bio PrimeSTAR* Max DNA Polymerase User Manual
This manual describes the PrimeSTAR* Max DNA Polymerase, a high‑speed, high‑accuracy enzyme for PCR applications. It explains the enzyme’s hot‑start formulation, recommended reaction components, and handling precautions.
Detailed sections cover recommended PCR cycling conditions, optimal template amounts, annealing temperatures, extension times, and troubleshooting tips, along with safety and licensing information for research‑use only. This manual is written in Japanese.
Covers: Contents · PCR Conditions · Optimal Parameter Settings · Features · Safety and Legal Notices
Latest Takara Bio manuals
Takara Bio RR096A One Step TB Green PrimeScript PLUS RT-PCR Kit User Manual
User Manual 16 pages · 195 KB · ENThe manual provides description, components, storage, protocol and troubleshooting for the Takara Bio RR096A One Step TB Green PrimeScript PLUS RT‑PCR Kit.
Takara Bio SMART-Seq HT PLUS Kit User Manual
User Manual 29 pages · 900 KB · ENUser manual for Takara Bio's SMART-Seq HT PLUS Kit, detailing protocols for cDNA synthesis and Illumina library preparation.
Takara Bio SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing User Manual
User Manual 20 pages · 591 KB · ENThe Takara Bio SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing User Manual provides step‑by‑step protocols for cDNA synthesis, amplification…
Takara Bio PrimeSTAR* Max DNA Polymerase User Manual
User Manual 12 pages · 325 KB · JAThe Takara Bio PrimeSTAR* Max DNA Polymerase manual provides usage instructions, optimal PCR parameters, and storage guidelines.
Takara Bio PrimeSTAR HS (Premix) R040 A User Manual v201702Da
User Manual 8 pages · 281 KB · ZHThe Takara Bio PrimeSTAR HS (Premix) R040 A user manual provides product description, composition, storage, PCR setup, optimization, troubleshooting, and…
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User Manual
Day-to-day operation, settings and troubleshooting. Start here if the product is already set up.
Questions answered in Takara Bio manuals
How many reactions can the SMART-Seq HT PLUS Kit perform?
The kit can prepare up to 48 reactions (catalog R400748) or 96 reactions (catalog R400749).
What are the recommended storage conditions for the kit reagents?
Control Total RNA should be stored at –70 °C, while all other kit components should be stored at –20 °C.
What amount of cells or RNA can be used with the kit?
The kit works with 1–1,000 cells or 10 pg–10 ng of total RNA in a 1–10 µl input volume.
From: Takara Bio SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing User Manual
Which sequencing platforms are supported?
The kit is compatible with Ion Torrent (PGM, Proton) and Illumina (HiSeq, MiSeq, NextSeq) library preparation kits.
From: Takara Bio SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing User Manual
What Mg2+ concentration should be used in the reaction?
Use 2 mM Mg2+ (2 X) as specified in the manual.
What is the recommended annealing temperature for PCR?
The recommended annealing temperature is 55 °C.