The Takara Bio SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing User Manual provides step‑by‑step protocols for cDNA synthesis, amplification…
This manual describes the SMART‑Seq® v4 Ultra Low Input RNA Kit for Sequencing, a kit that generates high‑quality, full‑length cDNA from very small numbers of cells or low amounts of total RNA. It outlines the required reagents, recommended equipment, and clean‑room practices needed to obtain reliable results.
The document is organized into sections covering the list of components, additional materials, general considerations, detailed protocols for first‑strand cDNA synthesis, LD‑PCR amplification, bead purification, validation, and library preparation for Illumina and Ion Torrent platforms, followed by references and appendices for magnetic bead handling and PCR optimization.
| Input cell range | 1–1,000 cells |
|---|---|
| Input RNA amount | 10 pg–10 ng total RNA |
| Input volume | 1–10 µl |
| Compatible sequencing platforms | Ion Torrent and Illumina platforms |
| cDNA synthesis time | approximately five hours |
| Total library construction time | within two working days |
The kit works with 1–1,000 cells or 10 pg–10 ng of total RNA in a 1–10 µl input volume.
The kit is compatible with Ion Torrent (PGM, Proton) and Illumina (HiSeq, MiSeq, NextSeq) library preparation kits.
The first‑strand cDNA synthesis can be completed in about five hours.