This manual provides instructions for the cultivation, observation, preservation, and quality complaint procedures for Qualityard's 293T human embryonic…
The document serves as a guide for researchers using Qualityard Biotechnology Co., Ltd.'s 293T human embryonic kidney cells. It details the cell line characteristics, including its origin from HEK 293 cells and expression of SV40 large T antigen. The manual outlines specific culture conditions requiring DMEM medium with 10% fetal bovine serum at 37°C in a CO2 incubator. This manual is written in Chinese.
The recommended medium is 90% DMEM plus 10% fetal bovine serum, cultured at 37°C with a gas phase of 95% air and 5% carbon dioxide.
Passaging is recommended when the cell density reaches between 85% and 95% confluence.
The recommended cryopreservation solution consists of 70% basal medium, 20% fetal bovine serum, and 10% DMSO.