This manual provides instructions for the cultivation, transportation, and maintenance of the Qualityard 5637 human bladder cancer cell line for research use.
The document serves as a guide for the Qualityard 5637 human bladder cancer cell line, detailing its origin, morphology, and basic culture conditions. It specifies that the cells are epithelioid and tumorigenic, requiring a medium of 90% RPMI1640 supplemented with 10% fetal bovine serum, incubated at 37°C in an atmosphere of 95% air and 5% CO2. The manual also notes that all tumor cells are considered to have potential biological hazards and must be handled in a Class II biological safety cabinet. This manual is written in Chinese.
| Origin | 68-year-old white male bladder cancer |
|---|---|
| Morphology | Epithelioid |
| Culture Medium | 90% RPMI1640 + 10% Fetal Bovine Serum |
| Gas Phase | 95% Air, 5% CO2 |
| Centrifugation Force | 80g-120g |
The cells require a medium of 90% RPMI1640 with 10% fetal bovine serum, incubated at 37°C in 95% air and 5% CO2.
Cells are typically shipped by digesting with trypsin, centrifuging, and shipping with serum, or by shipping frozen vials.
Passaging should be performed when the cell density reaches 85-95% confluence.
The freezing solution consists of 70% basal medium, 20% fetal bovine serum, and 10% DMSO.