This manual provides instructions for the culture, transport, observation, and storage of the Qualityard A-375 human melanoma cell line for research use.
The document serves as a guide for the Qualityard A-375 human melanoma cell line, detailing its origin, basic culture conditions, and safety precautions. It specifies that the cells are derived from a 54-year-old female and are suitable for research purposes only, requiring operation within a Class II biosafety cabinet. This manual is written in Chinese.
| Origin | 54-year-old female |
|---|---|
| Medium | 90% DMEM + 10% Fetal Bovine Serum |
| Atmosphere | 95% Air, 5% CO2 |
| Freezing Formula | 70% Base Medium + 20% FBS + 10% DMSO |
The recommended ratio for the first passage is 1:2.
Cells should be stored in liquid nitrogen.
Place the tube in a 37℃ water bath and shake until melted.