A user guide for the Qualityard A549/red human non-small cell lung cancer cell line, covering culture conditions, handling, and troubleshooting.
This manual provides instructions for the Qualityard A549/red human non-small cell lung cancer cell line, a stable transfected strain of the A549 cell line expressing red fluorescent protein. The document outlines basic culture conditions, including the use of F-12K medium supplemented with fetal bovine serum and PS, maintained at 37°C with 5% CO2. It also details safety precautions, noting that all tumor and virus-transfected cells are considered to have potential biological hazards and must be handled in a Class II biosafety cabinet. This manual is written in Chinese.
| Cell Line Origin | 58-year-old white male lung cancer tissue |
|---|---|
| Establishment Year | 1972 |
| Culture Medium | 89% F-12K + 10% Fetal Bovine Serum + 1% PS |
| Gas Phase | 95% Air, 5% Carbon Dioxide |
| Centrifugation Force | 80g-120g |
| Centrifugation Time | 2-5 minutes |
| Freezing Formula | 70% Base Medium + 20% Fetal Bovine Serum + 10% DMSO |
The cells should be cultured in 89% F-12K medium with 10% fetal bovine serum and 1% PS at 37°C in a gas phase of 95% air and 5% CO2.
Cells are transported either as live cells digested with trypsin and shipped in serum, or as frozen vials.
The first subculturing ratio is recommended to be 1:2, with later ratios of 1:4 to 1:6.
The freezing formula consists of 70% base medium, 20% fetal bovine serum, and 10% DMSO.