This manual provides usage instructions, culture conditions, and handling guidelines for Qualityard's A549/LUC human non-small cell lung cancer cells.
The document serves as a user guide for the A549/LUC human non-small cell lung cancer cell line provided by Qualityard Biotechnology Co., Ltd. It details the cell's origin, intended use for in vivo imaging via luciferase catalysis, and biosafety requirements for handling tumor cells. This manual is written in Chinese.
| Culture Medium Composition | 89% F-12K + 10% Fetal Bovine Serum + 1% PS |
|---|---|
| Gas Phase Composition | 95% Air, 5% Carbon Dioxide |
| Centrifugation Force for Resuspension | 80g-120g |
| Cryopreservation Formula | 70% Base Medium + 20% Fetal Bovine Serum + 10% DMSO |
The cells were established in 1972 from lung tissue of a 58-year-old white male.
It is a stable transfectant used for in vivo imaging via bioluminescence catalyzed by luciferase.
Cells should be passaged when they reach a density of 85-95% confluence.
The initial passaging ratio should be 1:2.