User guide for the LS-50B Luminescence Spectrometer covering safety, installation, operation, and maintenance.
This manual provides detailed instructions for safely operating and maintaining the LS-50B Luminescence Spectrometer. It covers installation procedures, accessory setup, safety precautions, and technical commands for system control. The guide also includes specifications, maintenance tips, and troubleshooting information for optimal instrument performance.
The LS-50B is a computer-controlled ratioing luminescence spectrometer capable of measuring fluorescence, phosphorescence, chemiluminescence, and bioluminescence. It features a xenon discharge lamp, gated photomultipliers, and Monk-Gillieson monochromators for precise wavelength control and spectral analysis.
| Principle | Computer controlled ratioing luminescence spectrometer |
|---|---|
| Source | Xenon discharge lamp, equivalent to 20 kW for 8 µs duration |
| Sample detector | Gated photomultiplier with modified S5 response for operation up to around 650 nm |
| Wavelength accuracy | + 1.0 nm |
| Wavelength reproducibility | + 0.5 nm |
| Excitation range | 200-800 nm with zero order selectable |
| Emission range | 200-650 nm with standard photomultiplier, 200-900 nm with optional R928 photomultiplier |
The wavelength accuracy is ±1.0 nm and the reproducibility is ±0.5 nm.
The instrument operates correctly indoors at ambient temperatures between 15 °C and 35 °C with a relative humidity of 75% maximum, non-condensing.
The wavelength accuracy is + 1.0 nm and the wavelength reproducibility is + 0.5 nm.
The minimum total period for phosphorescence mode is 13.0 ms (50 Hz).