User manual for the abcam ab178013 Human IL-6 SimpleStep ELISA Kit, detailing reagents, preparation, and assay procedures for quantitative measurement.
This document provides instructions for the abcam ab178013 Human IL-6 SimpleStep ELISA Kit, a quantitative measurement tool for Human IL-6 in serum, plasma, and cell culture supernatant. The kit is designed for research use only and includes all necessary reagents for the assay.
| Target Analyte | Human IL-6 protein |
|---|---|
| Sample Types | Human serum, plasma, and cell culture supernatant |
| Minimum Detectable Dose | 1.6 pg/mL |
| Plate Format | 96 Wells |
| Incubation Temperature | Room temperature (18 -25°C) |
| Wavelength | 450 or 600 nm |
No, the kit is for research use only and not intended for diagnostic purposes.
The dynamic range varies by sample type, from 1:3200 for cell culture supernatant to ≤50% for plasma and serum.
No, the kit does not show cross-reactivity with recombinant mouse IL-6.