This manual provides instructions for the Abcam ab83355 ATP Assay Kit, a colorimetric/fluorometric kit for measuring ATP in cell lysates, biological fluids…
The Abcam ab83355 ATP Assay Kit is a robust method designed to quantify total ATP in cell and tissue lysates, biological fluids, and blood cells. The assay utilizes the phosphorylation of glycerol to generate a product quantifiable either colorimetrically (OD 570 nm) or fluorometrically (Ex/Em = 535/587 nm). The fluorometric method offers 10-100 fold higher sensitivity than the colorimetric method, capable of detecting as low as 1 µM of ATP.
| Detection Limit (Fluorometric) | 1 µM |
|---|---|
| Colorimetric Wavelength | OD 570 nm |
| Fluorometric Excitation/Emission | Ex/Em = 535/587 nm |
| Sensitivity Ratio | 10-100 fold |
| Storage Temperature (Before Prep) | -20°C |
| Storage Duration (Unreconstituted) | 1 year |
| Storage Duration (Reconstituted) | 2 months |
The kit is designed for the measurement of ATP in cell and tissue lysates, biological fluids, and blood cells.
The fluorometric assay is 10-100 fold more sensitive than the colorimetric assay.
The kit should be stored at -20°C in the dark upon receipt.