Abcam ab139485 Golgi/ER Staining Kit provides a rapid method for visualizing Golgi and ER within living cells using Organelle Reagent III.
The Golgi/ER Staining Kit (ab139485) from Abcam provides a rapid and convenient method for visualizing the Golgi and endoplasmic reticulum (ER) within living cells. It is designed to work without requiring lengthy transfection procedures and is validated with various cell lines including HeLa, Jurkat, MDCK, and U2OS. The kit is suitable for identifying agents that perturb Golgi bodies and the ER, making it a useful tool for examining molecular transport and recycling in cellular secretory pathways.
The kit contains Organelle Reagent III, which includes a mixture of GA-selective, ER-selective, and nucleus-selective dyes suitable for live cell staining. The green dye component is noted for faithful localization to the Golgi apparatus with minimal ER staining, while the red dye stains the ER with high fidelity specifically designed for use with green fluorescing probes.
| Sample type | Adherent cells, Suspension cells |
|---|---|
| Assay type | Cell-based |
| Species reactivity | Mammals, Other species |
| Platform | Fluorescence microscope |
| Storage instructions | Please refer to protocols |
| Component 1 | 100 tests 10X Assay Buffer 1 |
| Component 2 | 50X Assay Buffer 2 |
| Component 3 | Organelle Reagent III (lyophilized) |
It provides a rapid and convenient method for visualizing the Golgi and ER within living cells, without requiring lengthy transfection procedures.
The kit is validated with HeLa, Jurkat, MDCK, and U2OS cell lines.
It contains a mixture of GA-selective, ER-selective, and nucleus-selective dyes suitable for live cell staining.