Abcam ab65393 is a colorimetric LDH Assay Kit for detecting cell cytotoxicity and plasma membrane damage using WST in cell culture media.
The Abcam ab65393 LDH Assay Kit (Cytotoxicity) is a colorimetric product designed for the fast and sensitive detection of Lactate Dehydrogenase (LDH) released from damaged cells. It is used to assess the level of plasma membrane damage in a cell population, serving as a marker for cell death or cytotoxicity. The assay relies on an enzymatic coupling reaction where LDH oxidizes lactate to generate NADH, which then reacts with the WST substrate to produce a yellow color. The intensity of this color correlates directly with the number of lysed cells.
| Detection method | Colorimetric |
|---|---|
| Sample type | Cell culture media |
| Assay type | Enzyme activity (quantitative) |
| Assay time | 1h 00m |
| Required volume | 10µl of culture medium |
| Storage temperature | -20°C |
The assay uses an enzymatic coupling reaction where LDH oxidizes lactate to generate NADH, which then reacts with WST to generate a yellow color.
Yes, since WST is very stable, the reaction can be read multiple times and can be stopped at any time point during the reaction.
No, cells can be cultured in regular 10% serum containing medium; no reducing serum or special medium is required.