Abcam ab213880 Mouse Neuropilin-1 ELISA Kit User Manual

This manual provides instructions for the quantitative measurement of Mouse Neuropilin-1 in various biological samples using a sandwich ELISA kit.

Brand: Abcam
Document type: User Manual
Language: English
Pages: 24
File format: PDF
File size: 122 KB
Published: 15 October, 2026
Updated: 15 October, 2026
MD5 checksum: A59F0D10A9F7548F1C6F3A6927450F9A
PDF: Abcam ab213880 Mouse Neuropilin-1 ELISA Kit User Manual PDF

About this manual

The Abcam ab213880 Mouse Neuropilin-1 ELISA Kit is designed for the quantitative detection of Mouse Neuropilin-1 (NRP1) in cell culture supernatants, cell lysates, tissue homogenates, serum, and plasma. The assay utilizes sandwich technology with a rat monoclonal antibody precoated on 96-well plates and a biotinylated goat polyclonal antibody for detection.

What's inside

Overview
Protocol Summary
Precautions
Storage and Stability
Materials Supplied
Reagent Preparation
Standard Preparation
Sample Preparation
Assay Procedure

Specifications

Detection Method	Sandwich ELISA technology
Coating Antibody	Rat monoclonal antibody
Plate Format	96-well plate
Standard Concentration	50,000 pg/mL stock solution
Read Wavelength	450 nm

Frequently asked questions

What samples can be tested with this kit?

The kit is designed for the quantitative measurement of Mouse Neuropilin-1 in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

Is this kit intended for diagnostic use?

No, the product is for research use only and is not intended for diagnostic use.

What equipment is required to perform the assay?

You need a microplate reader capable of measuring absorbance at 450 nm, an automated plate washer, multi- and single-channel pipettes, clean tubes, Eppendorf tubes, and washing buffer.

Download PDF (122 KB)