This datasheet describes the Abcam Mouse Heme Oxygenase 1 ELISA Kit (ab204524), a single-wash 90-minute sandwich ELISA for quantitative measurement of HO-1…
The Mouse Heme Oxygenase 1 ELISA Kit (ab204524) is a single-wash 90-minute sandwich ELISA designed for the quantitative measurement of Heme Oxygenase 1 protein in cell culture extracts, supernatant, serum, tissue extracts, urine, and plasma. It utilizes Abcam's proprietary SimpleStep ELISA® technology, which employs capture antibodies conjugated to an affinity tag recognized by the monoclonal antibody used to coat the plates. This approach allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time.
| Detection Method | Colorimetric |
|---|---|
| Precision Intra-assay CV% | 2.4% |
| Precision Inter-assay CV% | 2.6% |
| Sensitivity | 10 pg/ml |
| Range | 78.1 pg/ml - 5000 pg/ml |
| Assay Time | 1h 30m |
| Assay Duration | One step assay |
| Species Reactivity | Mouse |
| Components | 1 x 96 tests |
The kit is suitable for cell culture supernatant, urine, serum, tissue extracts, and plasma.
No, the kit does not react with human samples; reactivity was determined to be less than 3%.
The kit should be stored at +4°C.