Phire Animal Tissue Direct PCR Kit is a kit for direct PCR of non-fixed animal tissue samples without prior DNA purification. The kit contains reagents and tools for both direct and dilution methods.
The Thermo Phire Animal Tissue Direct PCR Kit enables direct PCR amplification from animal tissue without prior DNA purification. The kit includes Phire hot‑start DNA polymerase, 2× PCR buffer, dilution buffer, DNARelease additive, Harris Uni‑Core sampling tool, and control primers.
The manual provides detailed protocols for both direct and dilution methods, guidelines for sample handling with the Harris tools, recommended cycling conditions, and a troubleshooting guide to help resolve common amplification issues. This manual is written in Chinese.
| Kit model number | F-140 |
|---|---|
| Reactions per kit | 200 |
| Storage temperature | -20°C |
Add the tissue sample directly into the PCR reaction mixture (50 µL system) rather than into an empty tube.
After each sample, immerse the cutter tip in 2 % sodium hypochlorite solution, flush the piston, then dry with a clean paper towel; also rinse the slice pad in the same solution.
Store the kit components at –20 °C; the kit remains stable for one year when kept frozen.