The NanoDrop 2000/2000C spectrophotometer is a spectrophotometer produced by Thermo Scientific. It can detect 0.5-2ul samples with high accuracy and repeatability. ND2000C not only provides the convenience of NanoDrop sample retention patented technology, but also can use traditional cuvettes to detect samples.
The NanoDrop 2000/2000C spectrophotometer from Thermo Scientific provides micro‑volume UV‑Vis measurements with a wavelength range of 190‑840 nm. It supports both the proprietary sample‑holding pedestal and traditional cuvette detection, allowing analysis of nucleic acids, proteins and other biomolecules with sample volumes as low as 0.5 µL.
The manual explains hardware installation, USB connection, software installation on Windows XP/Vista, sample loading procedures, blank calibration, and specific application modes such as protein Pierce 660 nm and cell culture measurements. Maintenance tips, cleaning instructions and troubleshooting guidance are also included. This manual is written in Chinese.
| Instrument type | Spectrophotometer |
|---|---|
| Minimum sample volume | 0.5 µL |
| Wavelength range | 190‑840 nm |
| Light source | Xenon flash lamp |
| Detector | 2048‑pixel linear silicon CCD array |
| Wavelength accuracy | ±1 nm |
| Spectral resolution | ≤1.8 nm (FWHM @ 253.7 nm Hg) |
| Detection limit (dsDNA) | 2 ng/µL |
| Maximum concentration (dsDNA) | 15 000 ng/µL |
| Detection time | < 5 s (pedestal), < 3 s (cuvette) |
| Power consumption | 12‑18 W (max 30 W) |
The pedestal mode can measure as little as 0.5 µL, while the cuvette mode typically uses 1‑2 µL of sample.
It operates over a full UV‑Vis range from 190 nm to 840 nm.
Load a blank onto the pedestal, lower the arm, click the Blank button, and repeat until the absorbance variation is less than 0.04 A.
The software runs on 32‑bit Windows XP and Windows Vista.