TrueCut™ Cas9 Protein (Prototype) is a recombinant Streptococcus pyogenes Cas9 (wt) protein, purified from E. coli, for genome editing with CRISPR technology. Cas9 protein forms a very stable ribonucleoprotein (RNP) complex with the guide RNA (gRNA) component of the CRISPR/Cas9 system. Incorporation of nuclear localization signals (NLS) aids delivery to the nucleus, increasing the rate of genomic DNA cleavage. This protein is manufactured with aseptic filling, bioburden and endotoxin testing, and provided at a 10 mg/mL concentration in a transfection-ready format for electroporation.
