RayBio® Mouse Osteopontin IQELISA Kit is a modified ELISA assay with high sensitivity qPCR readout for the quantitative measurement of Mouse Osteopontin in serum, plasma, and cell culture supernatants.
This document provides detailed instructions for using the RayBio® Mouse Osteopontin IQELISA Kit, which combines ELISA specificity with qPCR sensitivity. The assay uses an antibody-coated PCR plate to capture Osteopontin, followed by detection and quantification via real-time PCR. The protocol includes reagent preparation, assay steps, data analysis, and troubleshooting guidance.
The kit requires a real-time PCR instrument, precision pipettes, and standard materials for dilution and washing. Reagents must be prepared and stored according to specified conditions, and samples are incubated with standards, detection antibodies, and PCR components before running the qPCR cycle.
| Minimum Quantifiable Dose | 0.49 pg/ml |
|---|---|
| Recovery Rate | 93% |
| Inter-Plate CV | Below 15% |
The minimum quantifiable dose is typically 0.49 pg/ml.
The recovery rate is 93%, with a range from 84% to 100%.