A user manual for RayBio® Mouse MCP-1 IQELISA Kit
The RayBio Mouse MCP-1 IQELISA Kit is a modified ELISA assay with a high-sensitivity qPCR readout designed for the quantitative measurement of Mouse MCP-1 in serum, plasma, and cell culture supernatants. The assay utilizes an antibody specific for Mouse MCP-1 coated on a 96-well PCR plate, followed by a detection step and real-time PCR amplification to determine antigen concentration based on Ct values.
The document details the reagents included in the kit, storage conditions, and additional materials required such as a real-time PCR instrument. It provides step-by-step instructions for reagent preparation, sample dilution, and the complete assay procedure, including incubation times and temperatures. Finally, it explains how to calculate results using standard curves and provides troubleshooting guidance.
| Minimum quantifiable dose | 0.488 pg/ml |
|---|---|
| Sample types | serum, plasma, and cell culture supernatants |
| Plate type | 96-well PCR plate |
| Storage temperature | 2° to 8°C |
| Standard storage temperature | -20°C or -80°C |
| Incubation time (Step 1) | 2.5 hours at room temperature or overnight at 4°C |
| Incubation time (Step 2) | 1 hour at room temperature |
| Incubation time (Step 3) | 1 hour |
| Incubation time (Step 4) | 5 minutes |
| PCR activation | 3 minute activation at 95°C |
The minimum quantifiable dose of MCP-1 is typically 0.488 pg/ml.
This kit is designed for the quantitative measurement of Mouse MCP-1 in serum, plasma, and cell culture supernatants.
The kit may be stored for up to 6 months at 2° to 8°C from the date of shipment.
The standard should be stored at -20°C or -80°C, with -80°C being recommended.