The RayBio® Mouse Lymphotactin IQELISA Kit is a modified ELISA assay with high sensitivity qPCR readout for the quantitative measurement of Mouse Lymphotacin (XCL1) in serum, plasma, and cell culture supernatants.
This document provides step-by-step instructions for using the RayBio® Mouse Lymphotactin IQELISA Kit, which combines ELISA specificity with qPCR sensitivity. It includes reagent preparation, assay procedure, and data calculation using Ct values. The kit is designed for measuring Mouse Lymphotacin (XCL1) in serum, plasma, and cell culture supernatants.
The manual details storage conditions, required materials, and troubleshooting guidance. It also explains how to prepare standards, dilute samples, and run real-time PCR with specific instrument settings and buffer dilutions.
| Minimum Quantifiable Dose | 2.9 pg/ml |
|---|---|
| Recovery Rate | 78% |
| Intraplate CV | below 10% |
| Interplate CV | below 15% |
| PCR Plate Type | 0.2 mL, non-skirted 96-well plate (ThermoFisher, cat. no.: AB0600) |
The minimum quantifiable dose is typically 2.9 pg/ml.
The recovery rate is 78%, with a range from 68% to 87%.
Intraplate CV is below 10% and interplate CV is below 15%.