This is a manual of ELISA kit
This document provides step-by-step instructions for using the LSBio Guinea pig STS / ASC / Steroid Sulfatase ELISAKit based on the sandwich ELISA principle. It details kit components, storage conditions, required equipment, assay procedure, calculation of results, and troubleshooting. The kit is for research use only and not approved for diagnostic or clinical use. Safety precautions regarding hazardous reagents are included.
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The assay is based on the sandwich ELISA principle, using a capture antibody, detection antibody, and HRP-conjugated avidin to detect the target antigen.
Upon receipt, store the kit at 4°C if used within 24 hours. Otherwise, store the Assay Plate, Standard, Detection Reagent A, and Detection Reagent B at -20°C. Store all other components at 4°C. TMB Substrate should never be frozen.
A microplate reader with 450nm wavelength filter, high-precision pipette, sterile pipette tips, Eppendorf tubes, 37°C incubator, deionized or distilled water, and absorbent paper.