This is a manual for a LPA / Lipoprotein a ELISAKit. The kit is for research use only.
This document provides detailed instructions for using the Rabbit LPA / Lipoprotein a ELISAKit (Sandwich ELISA) from LSBio. It covers assay principles, kit components, storage, required supplies, experimental layout, sample preparation, assay procedure, calculation of results, and troubleshooting. The kit is for research use only and not approved for diagnostic use. It includes storage instructions, reagent preparation steps, and guidance for handling samples and interpreting results.
The manual also includes a troubleshooting guide for common issues such as poor standard curves, low signal, high background, and low sensitivity, with suggested solutions. It emphasizes proper storage, pipetting accuracy, and validation steps before using valuable samples. Technical support is available via email.
The optical density of the well is measured at a wavelength of 450 nm ± 2 nm.
The Assay Plate should be stored at -20°C if not intended for use within 24 hours.
It is recommended that the kit be used within 1 month once individual reagents are opened.