The document is the user manual of a kit named Chicken FOXC1 ELISA Kit.
This document provides step-by-step instructions for using the Chicken FOXC1 ELISA Kit based on the sandwich ELISA principle. It includes details on kit components, storage conditions, required equipment, assay planning, sample preparation, and troubleshooting common issues. The kit is intended for research use only and not for diagnostic purposes.
The manual covers experimental layout, standard and sample preparation, reagent preparation, assay procedure, result calculation, and includes a troubleshooting guide for common problems such as poor standard curves or low signal.
| Target | Chicken FOXC1 |
|---|---|
| Detection Method | Colorimetric - 450nm |
| Storage Conditions | Assay Plate, Standard, Detection Reagent A, and Detection Reagent B stored at -20°C; other components at 4°C; TMB Substrate never frozen. |
| Intended Use | Research Use Only. Not for Diagnostic Use. |
The kit uses a colorimetric detection method at 450nm wavelength.
Store the Assay Plate, Standard, Detection Reagent A, and Detection Reagent B at -20°C. Store all other components at 4°C. Never freeze the TMB Substrate.
No, this kit is for Research Use Only and is not approved for diagnostic or clinical use.
Check pipettes for accuracy and ensure the standard is properly dissolved by gently mixing the vial.