LSBio Mouse UCHL1 / PGP9.5 ELISAKit is a research use ELISA kit for research of UCHL1 and PGP9.5 expression. The kit is based on sandwich ELISA principle and can detect UCHL1 and PGP9.5 expression level in 2 hours.
This document is the user manual for the LSBio Mouse UCHL1 / PGP9.5 ELISA Kit, catalog number LS-F18413. It details the sandwich ELISA assay principle, kit components, and storage requirements. The kit is intended for research use only and is not approved for diagnostic use in humans or clinical diagnosis.
The manual outlines the complete experimental procedure, including sample collection and preparation for various biological samples such as plasma, serum, and cell lysates. It provides guidance on experimental layout, reagent preparation, and the step-by-step assay procedure. Additionally, it includes a troubleshooting guide to address common issues like poor standard curves, low signal, and high background noise.
| Target | Mouse UCHL1 / PGP9.5 |
|---|---|
| Detection Method | Colorimetric - 450nm |
| Plate Type | Coated 96-well Strip Plate |
| Standard Quantity | 2 vials |
| Sample Diluent Volume | 1 vial x 20 ml |
| Assay Diluent A Volume | 1 vial x 12 ml |
| Assay Diluent B Volume | 1 vial x 12 ml |
| Detection Reagent A Volume | 1 vial x 120 µl |
| Detection Reagent B Volume | 1 vial x 120 µl |
| Wash Buffer Quantity | 1 vial x 20 ml (30 x) |
| TMB Substrate Volume | 1 vial x 9 ml |
| Stop Solution Volume | 1 vial x 6 ml |
No, this kit is for Research Use Only and is not approved for use in humans or for clinical diagnosis.
The optical density (OD) of the well is measured at a wavelength of 450 nm ± 2 nm.
Upon receipt, the kit should be stored at 4 ° C if intended for use within 24 hours.
Check pipettes for accuracy and ensure the standard powder is dissolved thoroughly by a gentle mix.