This manual provides instruction of using GlobalFiler™Express PCR Amplification Kit
The Applied Biosystems GlobalFiler Express PCR Amplification Kit is a 6-dye, short tandem repeat (STR) multiplex assay designed for the amplification of human genomic DNA. It targets 21 autosomal STR loci, 1 Y-STR, 1 Y chromosome insertion/deletion marker, and Amelogenin, delivering a 24-locus multiplex with high discrimination power and sensitivity. The kit is intended for research, forensic, or paternity use only and is not for diagnostic procedures.
| Loci amplified | 21 autosomal STR loci, 1 Y-STR, 1 Y indel, Amelogenin |
|---|---|
| Amplification time | ~45 minutes |
| Dye configuration | 6-dye |
| Intended use | Research, Forensic, or Paternity Use Only |
No, this kit is for research, forensic, or paternity use only and is not for use in diagnostic procedures.
The highly optimized buffer formulation allows completion of amplification in approximately 45 minutes.
Data is analyzed using GeneMapper ID-X Software.