Thermo Fisher Scientific Yfiler Plus PCR Amplification Kit User Guide

Yfiler ™ Plus PCR Amplification Kit is a PCR amplification kit


Thermo Fisher Scientific Yfiler Plus PCR Amplification Kit User Guide - cover page
Brand
Thermo Fisher Scientific
Category
Other
Document type
User Manual
Language
English
Pages
131
File format
PDF
File size
2.9 MB
Published
15 October, 2023
Updated
15 October, 2023
MD5 checksum
D1F68CE8C85DAE2E12169658B5C4DFDA

About this manual

The Applied Biosystems Yfiler Plus PCR Amplification Kit is a 6-dye, short tandem repeat (STR) multiplex assay optimized for amplifying 27 Y-STR loci from male-specific sample types. It supports direct amplification from single-source samples such as blood and buccal samples on treated paper substrates, blood on untreated paper substrates treated with Prep-n-Go Buffer, and buccal samples on swab substrates treated with Prep-n-Go Buffer. The kit utilizes non-nucleotide linkers in primer synthesis to ensure reproducible allele positioning and clean electrophoretic backgrounds.

What's inside

  • Product description
  • Kit overview
  • Perform PCR
  • Perform electrophoresis
  • Analyze data with GeneMapper ID-X Software
  • Experiments and results
  • Troubleshooting
  • Safety

Specifications

Number of Y-STR loci amplified27
Dye system6-dye fluorescent system
Sample types supportedBlood and buccal samples on treated paper substrates
Compatible instruments3500 Series and 3130 Series Genetic Analyzers

Frequently asked questions

What are the catalog numbers for this kit?

The catalog numbers for the Yfiler Plus PCR Amplification Kit are 4484678 and 4482730.

Can this kit be used for diagnostic procedures?

No, this kit is for research, forensic, or paternity use only and is not for use in diagnostic procedures.

Which software is used for data analysis?

Data is analyzed using GeneMapper ID-X Software.

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