Agilent Poroshell 120 Columns for HPLC UHPLC Handbook

Agilent Poroshell 120 columns can enhance the performance of both standard and high-pressure liquid chromatography (HPLC), offering exceptional reproducibility, lower back pressure, and improved peak shape for the separation of small molecules and peptides.


Agilent Poroshell 120 Columns for HPLC UHPLC Handbook - cover page
Brand
Agilent
Document type
Product Leaflet
Language
French
Pages
28
File format
PDF
File size
5 MB
Published
05 July, 2012
Updated
29 September, 2023
MD5 checksum
774412DEDD97D26F53268D29C7D69BFD

About this manual

Agilent Technologies presents the Poroshell 120 column series as a new generation of chromatography columns designed to improve efficiency and resolution. These columns utilize a unique single-step coacervation process to create particles with a solid core and a porous shell, offering superior performance for separating small molecules and peptides. This manual is written in French.

What's inside

  • Why Poroshell 120 Columns are Different
  • Manufacturing Process of Surface-Porous Particles
  • High Efficiency at Lower Pressure
  • Improving HPLC Instrument Performance
  • Increasing UHPLC Method Flexibility
  • Easy Method Transfer from 5 um to Poroshell 120
  • New Options for Proteins and Peptides
  • Agilent 1200 Infinity Systems

Specifications

Pressure CapabilityUp to 1200 bar (UHPLC)
Particle Size Options2.7 um and 3.5 um available

Frequently asked questions

What is the main difference in manufacturing for Poroshell 120 columns?

They are made in a single step using a coacervation process, unlike the classic multi-layer method.

How does the particle structure improve efficiency?

The solid core allows for tighter packing and higher efficiency compared to fully porous particles of similar size.

Which phase is recommended for peptide mapping?

The EC-C18 phase (postsilanized) is the first choice for most separations including peptide maps.

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