Agilent BioSeparation Selection Guide

About this manual

This document serves as a selection guide for Agilent products used in bioseparation. It provides strategies for developing reversed-phase HPLC methods for proteins and peptides, detailing column choices based on molecular weight and pH conditions.

The guide covers various column technologies including ZORBAX StableBond, Poroshell 300SB, and Bio-Monolith columns. It includes specific recommendations for analytical separations, LC/MS applications, and high-sensitivity capillary chromatography. This manual is written in German.

What's inside

• Reversed Phase-HPLC-Biosaulen
• Auswahl der Startersaule und Anfangsbedingungen
• Optimierung der Léslichkeit der Probe
• Erhéhen der Temperatur
• Optimierung des pH-Werts der mobilen Phase
• Trennungen von Proteinen und Peptiden mit LC/MS-Methoden
• Hohe Empfindlichkeit mit Kapillarsaulen
• Saulenspezifikationen

Specifications

Frequently asked questions

Which column is recommended as a starting point for peptide and protein analysis?

The StableBond 300SB-C8 column is widely recommended as the first choice due to its excellent lifetime in TFA-containing solvents.

What mobile phase is typically used for reversed-phase separations of proteins and peptides?

A water/acetonitrile gradient containing 0.1% TFA is commonly used to elute all relevant components.

Which column should be used for separations at neutral or high pH values?

The ZORBAX 300Extend-C18 column is an excellent choice for separations performed at a medium or high pH value.

What is the recommended particle size for Poroshell columns?

The specifications list a particle size of 5 µm for the various Poroshell column models.