The paper introduces SemiQuant method, which is used for preliminary identification of unknown compounds based on mass spectral features, retention time and chemical criteria such as sample extraction and processing. The method can be used to estimate the concentrations of various similar compounds.
The document introduces the SemiQuant software approach used by analysts conducting GC/MS determinations to estimate quantities of non-target or unknown compounds. It explains that this feature is tailored for approximations where a standard has not yet been obtained, utilizing Retention-Time Locked (RTL) methods to an internal standard or analyte.
| Software Requirement | GC/MS ChemStation SW G1701DA Enhanced mode only |
|---|---|
| Response Factor Range | 0.1 to 10 |
It denotes the estimation of an uncalibrated compound's amount or concentration by comparison to a calibrated, known compound analyzed under the same method.
Execute the 'Convert Target Compounds to Semi-Quant Compounds' menu item in the Data Analysis package under Calibration.
Add the compound as a SemiQuant compound, review a calibration report to determine an estimated response factor (default 1.00 if unknown), and use 'Create Responses for new SemiQuant Compounds' to enter the relative response factor.